Table 1 Effects of different batches of vectors and particles on CD34+ cells
From: Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
Integrating lentiviral vector LV | Integrase-deficient particles dINT | Transgene construct- deficient particles dGEN | Envelope-deficient particles dENV | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Batches tested | LV1 | LV2 | LV3 | LV4 | LV5 | dINT1 | dINT2 | dGEN1 | dGEN2 | dENV1 | dENV2 | dENV3 |
Number of times tested independently | 2 | 1 | 2 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 1 |
Number of different CD34+ cell donors tested | 6 | 3 | 6 | 6 | 3 | 6 | 3 | 6 | 3 | 6 | 3 | 3 |
Average transduction efficiency (% GFP + cells ± SD) (range) | 54 ± 8 (41–64) | 52 ± 13 (39–7) | 59 ± 6 (47–72) | 57 ± 14 (39–85) | 41 ± 7 (31–47) | 0.1 ± 0.1 (0–0.3) | 0.2 ± 0.2 (0–0.7) | 0.1 ± 0.1 (0–0.1) | 0 ± 0 (0–0.1) | 0 ± 0 (0–0.3) | 0 ± 0 (0–0.1) | 0 ± 0 (0–0.1) |
Average vector copy number per cell (VCN ± SD) | 3.3 ± 1.1 | NT | 2.6 ± 1.3 | 0.4 ± 0.02 | 0.7 ± 0.1 | 0 ± 0 | 0.1 ± 0.1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
Increased methylation cluster effecta | Low, Low | High | High, High | Low, Low | Low | High, High | High | Low, High | High | Low, Low | Low | Low |
Decreased methylation cluster effecta | Low, Low | Low | Low, Low | Low, Low | Low | Low, High | Low | Low, High | Low | Low, Low | Low | Low |