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Table 1 Effects of different batches of vectors and particles on CD34+ cells

From: Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration

  Integrating lentiviral vector LV Integrase-deficient particles dINT Transgene construct- deficient particles dGEN Envelope-deficient particles dENV
Batches tested LV1 LV2 LV3 LV4 LV5 dINT1 dINT2 dGEN1 dGEN2 dENV1 dENV2 dENV3
Number of times tested independently 2 1 2 2 1 2 1 2 1 2 1 1
Number of different CD34+ cell donors tested 6 3 6 6 3 6 3 6 3 6 3 3
Average transduction efficiency (% GFP + cells ± SD) (range) 54 ± 8 (41–64) 52 ± 13 (39–7) 59 ± 6 (47–72) 57 ± 14 (39–85) 41 ± 7 (31–47) 0.1 ± 0.1 (0–0.3) 0.2 ± 0.2 (0–0.7) 0.1 ± 0.1 (0–0.1) 0 ± 0 (0–0.1) 0 ± 0 (0–0.3) 0 ± 0 (0–0.1) 0 ± 0 (0–0.1)
Average vector copy number per cell (VCN ± SD) 3.3 ± 1.1 NT 2.6 ± 1.3 0.4 ± 0.02 0.7 ± 0.1 0 ± 0 0.1 ± 0.1 0 ± 0 0 ± 0 0 ± 0 0 ± 0 0 ± 0
Increased methylation cluster effecta Low, Low High High, High Low, Low Low High, High High Low, High High Low, Low Low Low
Decreased methylation cluster effecta Low, Low Low Low, Low Low, Low Low Low, High Low Low, High Low Low, Low Low Low
  1. aSee Fig. 2b