Fig. 2
5hmC enrichment in gene bodies positively correlates with gene expression and active chromatin. Heatmaps showing the enrichment of the indicated epigenetic mark −1.5-kb upstream of the TSS to +1.5-kb downstream of the TTS in all annotated human genes ordered by decreasing expression (log2-transformed FPKM values) in CD34+ (a), T cells (b), monocytes (c) and B cells (d). The Spearman rank (ρ) correlation coefficient below the heatmaps indicates the correlation between gene body 5hmC levels and gene expression (log2 FPKM) or a specific histone modification (exact permutation test for testing the null hypothesis of no correlation, two-tailed, *p < 5 × 10−20). Averaged 5hmC enrichment in CD34+ (e), T cells (f), monocytes (g) and B cells (h) 5-kb downstream of the TSS of genes expressed at high, low or intermediate level. Genes were divided into tertiles based on expression levels and averaged 5hmC enrichment 5-kb downstream of the TSS. Differences in 5hmC enrichment between the tertiles were assessed using the Kolmogorov–Smirnov test (*; #significant differences in 5hmC enrichment with D > 0.45 and p < 10−10. *High versus intermediate or low gene expression; #intermediate versus low gene expression)