Fig. 1
From: Abnormal X chromosome inactivation and sex-specific gene dysregulation after ablation of FBXL10
a Domain organization of FBXL10-1 and FBXL10-2 proteins. b Intron–exon structure of Fbxl0-1 and Fbxl0-2 genes and Cre-mediated deletion of promoter and first exon of Fbxl10-2. LoxP sites introduced by homologous recombination are shown as blue arrowheads. The alternative first exon of Fbxl10-2 is not present in transcript of Fbxl10-1. Gene trap truncation (T allele) is a null allele of both Fbxl10-1 and Fbxl10-2 and is described in [9]. c Immunoblot confirmation that the deletion shown in b and denoted as Δ-2 prevents expression of FBXL10-2. The gene trap allele denoted as T eliminates both forms of FBXL10. d Craniofacial abnormalities in E12.5 embryos (top row) and E17.5 embryos (bottom row) homozygous for the Δ-2 allele. Compression of the anterior portion of the head is apparent (top and bottom rows), as is the eyes-open-at-birth phenotype (black arrowhead at bottom). A tortuous neural tube was observed in both T/T and Δ-2/Δ-2 embryos (middle row)