Fig. 8

Timing of replication initiation in shared and cell-type-specific origins. Groups of a EB shared, b EB cell-type-specific, c K562 shared and d K562 cell-type-specific replication origins were stratified according to replication time. Replicating quintiles were created from BED files based on TimEX replication timing data for the EB cells [51] and Repli-seq for the K562 cell line [62]. The frequency of replication initiation in the first, third and fifth quintiles was plotted for genomic regions flanking replication origins. The histogram x axis extends 5-kb upstream and 5-kb downstream from the center of shared or cell-type-specific replication origins. The y axis represents the number of peaks shared among the indicated samples. Data are summarized in the histogram (e). Bar graph depicting the percent of shared (left) and cell-type-specific (right) origins found in each replication timing period. Shared replication origins exhibited a slight preference for early replication, whereas cell-type-specific replication origins were enriched in late timing stages