Fig. 3From: Regulation of chaperone binding and nucleosome dynamics by key residues within the globular domain of histone H3Histone mutations result in decreased Spt2, Spt6, and Spt16, occupancy over highly transcribed, but not lowly transcribed, genes. a ChIP analysis was performed on chromatin prepared from strains expressing HHTS-HHFS alleles (YS454-YS456, YS493-YS495) or the indicated histone mutant alleles (YS458-YS462, YS465, YS471, YS472, YS474, YS504-YS506, YS518, YS519, YS521, YS525-YS527) that were grown in YPD at 30 °C. The amount of immunoprecipitated DNA was determined by qPCR and is shown as a percentage of the input material and represents the mean ± SEM of three biological replicate experiments. Factor occupancy was measured within the coding region of a highly transcribed gene, PYK1. The regions assayed by qPCR are marked with the black bars in the diagram provided for the gene. b Factor occupancy at TUB2, a lowly transcribed gene, was determined as described in a Back to article page