Fig. 8
From: The high mobility group protein HMO1 functions as a linker histone in yeast
Dynamic chromatin environment in hmo1∆ that leads to faster chromatin remodeling and DSB repair is restored to wild-type levels by expression of H1. a Survival of DDY3, hmo1∆, hmo1-AB and the corresponding strains expressing H1. After DSB induction, cells were plated and colonies counted. Three independent experiments were performed. Error bars represent standard deviation. b Cells not induced to express HO were plated as control. c qRT-PCR analysis of ChIP using antibody to phosphorylated H2A, monitoring presence of γ-H2AX at MAT during DNA damage (galactose) and repair (glucose). Data are normalized to corresponding input control at each time point. d qRT-PCR analysis of ChIP using antibody to Arp5, monitoring presence at MAT (left panel) and 3.1 kb downstream of DSB (right panel) during DNA damage (galactose) and repair (glucose). Data are normalized to corresponding input control at each time point. Three independent experiments were performed. Error bars represent standard deviation. In all panels, asterisks represent statistical significance from DDY3 at the respective time points based on Student’s t test (P < 0.05)