Fig. 4
From: The high mobility group protein HMO1 functions as a linker histone in yeast
Effect of HMO1 or Hho1p on binding of the other protein. a Chromatin immunoprecipitation (ChIP) with DDY3 and hho1∆ using antibody to FLAG-tagged HMO1, monitoring binding at MAT, 18S rDNA, and KRE5. IC, input control; No, no antibody; IP, immunoprecipitation with anti-FLAG. b qRT-PCR analysis of ChIP data corresponding to (a). c ChIP with DDY3 and hmo1∆ using antibody to Hho1p, monitoring binding at MAT, 18S rDNA, and KRE5. d qRT-PCR analysis of ChIP data corresponding to (c). Data were normalized to corresponding input control at each time point. Fold enrichment = ChIP/Input DNA. Three independent experiments were performed. Error bars represent standard deviation. Asterisks represent statistical significance from DDY3 at the same locus based on Student’s t test (P < 0.05)