Fig. 1
From: The high mobility group protein HMO1 functions as a linker histone in yeast
Resistance of chromatin to nuclease digestion requires linker histone H1 or HMO1 containing its lysine-rich extension. a–c MNase digestion of chromatin isolated from wild-type cells (DDY3), hmo1∆, and hmo1-AB, respectively. d–f MNase digestion of chromatin isolated from wild-type, hmo1∆, and hmo1-AB cells expressing human linker histone H1.2 under control of a strong, constitutive promoter. Nuclei were digested with 0.25 U/µl MNase for the time indicated. Nucleosomal DNA was purified and resolved by agarose gel electrophoresis and stained with ethidium bromide