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Table 3 MS-MLPA analysis on the FMR1 locus before and after 7-day treatment with 5-azadC (T1) of three different normal control female cell lines (WTA, B and C)

From: Genome-wide methylation analysis demonstrates that 5-aza-2-deoxycytidine treatment does not cause random DNA demethylation in fragile X syndrome cells

Probes WTA UT ratio WTA T1 ratio WT B UT ratio WT B T1 ratio WTC UT ratio WTC T1 ratio
Prom 0.48 0.38 0.52 0.3 0.52 0.37
Prom 0.47 0.25 0.42 0.32 0.53 0.25
Exon 1 0.33 0.26 0.27 0.3 0.39 0.28
Intron 1 0.56 0.36 0.52 0.19 0.68 0.42
Intron 1 0.25 0.19 0.3 0.4 0.38 0.23
Intron 1 0.36 0.24 0.35 0.25 0.35 0.25
Intron 1 0.35 0.24 0.3 0.25 0.39 0.26
Exon 7 0.98 0.46 * 0.98 0.6 * 0.93 0.58 *
Exon 17 0.97 0.9 0.94 0.93 0.97 0.88
Exon 1 AFF2 0.52 0.45 0.54 0.43 0.61 0.42
Exon 1 AFF2 0.49 0.37 0.42 0.33 0.42 0.33
Exon 1 AFF2 0.23 0.23 0.3 0.19 0.34 0.24
Exon 1 AFF2 0.46 0.3 0.49 0.36 0.53 0.38
Exon 1 AFF2 0.27 0.17 0.22 0.14 0.38 0.23
Xp22 0.41 0.34 0.38 0 0 0
  1. Only methylation-sensitive probes are listed
  2. Note that the asterisks indicate that the magnitude of the probe ratio exceeds the set of arbitrary border values. The italic values represent levels of methylation lower than 30 %, bold italic values represent those between 30 and 70 %, while underlined values represent those higher than 70 %