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Table 2 MS-MLPA analysis of the imprinted locus Beckwith–Wiedemann/Silver–Russell (BWS/SRS) on chromosome 11 for WT1, WT2, FXS1 and FXS2 before and after treatment with 5-azadC [1 µM] at three different time points (T1, T3 and T8)

From: Genome-wide methylation analysis demonstrates that 5-aza-2-deoxycytidine treatment does not cause random DNA demethylation in fragile X syndrome cells

Probes WT1 UT ratio WT1 T1 ratio WT1 T3 ratio WT1 T8 ratio
11-002.0 H19 0 0 0 0
11-002.0 H19 0.49 0.24 0.26 0.51
11-002.0 H19 0.54 0.37 0.41 0.57
11-002.0 H19 0.49 0.28 0.32 0.55
11-002.1 IGF2 0 0 0 0
11-002.7 KCNQOT1 0.53 0.38 0.39 0.57
11-002.7 KCNQOT1 0.46 0.28 0.33 0.51
11-002.7 KCNQOT1 0.45 0.33 0.37 0.54
11-002.7 KCNQOT1 0.41 0.3 0.37 0.58
11-002.9 CDKN1c 0.17 0.18 0.2 0.15
Probes WT2 UT ratio WT2 T1 ratio WT2 T3 ratio WT2 T8 ratio
11-002.0 H19 0.23 0 0 0
11-002.0 H19 0.55 0.26 0.26 0.47
11-002.0 H19 0.54 0.37 0.35 0.5
11-002.0 H19 0.52 0.35 0.36 0.51
11-002.1 IGF2 0 0 0 0
11-002.7 KCNQOT1 0.48 0.41 0.29 0.59
11-002.7 KCNQOT1 0.44 0.33 0.22 0.52
11-002.7 KCNQOT1 0.42 0.34 0.26 0.5
11-002.7 KCNQOT1 0.42 0.35 0.18 0.56
11-002.9 CDKN1c 0.13 0.15 0 0.15
Probes FXS1 UT ratio FXS1 T1 ratio FXS1 T3 ratio FXS1 T8 ratio
11-002.0 H19 0.23 0.05 0.07 0.07
11-002.0 H19 0.54 0.1 0.09 0.1
11-002.0 H19 0.62 0.42 0.47< 0.59
11-002.0 H19 0.59 0.32 0.45 0.49
11-002.1 IGF2 0.02 0.03 0.02 0.02
11-002.7 KCNQOT1 0.59 0.42 0.54 0.6
11-002.7 KCNQOT1 0.59 0.33 0.44 0.54
11-002.7 KCNQOT1 0.51 0.42 0.53 0.5
11-002.7 KCNQOT1 0.54 0.36 0.49 0.58
11-002.9 CDKN1c 0.1 0.13 0.16 0.1
Probes FXS2 UT ratio FXS2 T1 ratio FXS2 T3 ratio FXS2 T8 ratio
11-002.0 H19 0 0 0.19 0
11-002.0 H19 0.26 0.22 0.23 0.29
11-002.0 H19 0.67 0.37 0.42 0.47
11-002.0 H19 0.71 0.47 0.34 0.77
11-002.1 IGF2 0.19 0.14 0.11 0.18
11-002.7 KCNQOT1 0.5 0.34 0.68 0.6
11-002.7 KCNQOT1 0.4 0.42 0.44 0.42
11-002.7 KCNQOT1 0.55 0.4 0.74 0.58
11-002.7 KCNQOT1 0.5 0.47 0.44 0.92
11-002.9 CDKN1c 0.25 0.28 0.3 0.35
  1. Only methylation-sensitive probes are listed
  2. The italic values represent levels of methylation lower than 30 %, bold italic values represent those between 30 and 70 %, while underlined values represent those higher than 70 %