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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Genome-wide methylation analysis demonstrates that 5-aza-2-deoxycytidine treatment does not cause random DNA demethylation in fragile X syndrome cells

Fig. 2

ChIP analysis of euchromatic and heterochromatic markers at the FMR1 locus. Quantification of IP-DNA by real-time PCR after immunoprecipitation with antibodies against euchromatic markers (H3K4me2 and H3K27me2) in the promoter (a, c) and exon 1 (b, d) regions of FMR1, respectively, positively correlated with the level of transcriptional reactivation observed by quantitative RT-PCR. Heterochromatic markers (H3K9me2 and H3K27me3) in the promoter (e, g) and exon 1 (f, h) regions of FMR1, respectively, proved to be relatively stable after treatment with 5-azadC even though the trend was in accordance with the transcriptional data. These results refer to two separate ChIP assay performed on cells from two independent experiments on FXS2 cell line at T1 and T6 (n = 2). The statistical analysis was conducted using the Kruskal–Wallis nonparametric test (p < 0.1)

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