Skip to main content
Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Mutations that prevent or mimic persistent post-translational modifications of the histone H3 globular domain cause lethality and growth defects in Drosophila

Fig. 6

Commercial H3 K122Ac antibodies are not specific. a Wing and eye imaginal discs with GFP-negative mutant clones. Merged images show the nuclear marker DAPI in blue, H3 K122Ac in magenta, and GFP+ and GFP− regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual H3 K122Ac channels. b Western blot analysis with the Abcam anti-H3 K122Ac antibody using acid-extracted histones collected from HEK293T cells transiently transfected with YFP-tagged WT or mutant histone H3 expression plasmid (H3.1 K115R, K122R, T118E, or K115R/K122R) shows that the anti-H3 K122Ac antibody non-specifically recognizes unmodified H3 and/or other modification(s) besides H3 K122Ac. c Immunoprecipitation (IP) analysis with the Abcam anti-H3 K122Ac antibody using nuclear extract from stable cell lines expressing Flag-tagged WT or mutant histone H3 (H3.1 K115R, K122R, or K115R/K122R) shows that the anti-H3 K122Ac antibody non-specifically recognizes unmodified histone H3 and/or other modification(s) besides H3 K122Ac

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935