Fig. 3

There is no overt changes in cell cycle or apoptosis markers in H3 T118 mutant clones. Wing imaginal discs with GFP-negative mutant clones generated using Ubx-FLP. a Merged images show the nuclear marker DAPI in blue, BrdU in magenta, and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual BrdU channels. Within each mutant, we looked at GFP⁻ clones within the zone of non-proliferation of the wing disc (see Additional file 1: Fig. S1) in search of consistent increases in BrdU incorporation. Conversely, we looked at GFP⁻ clones outside of the zone of non-proliferation to determine whether there was a consistent decrease of BrdU incorporation compared to their neighboring GFP⁺ control cells. b Merged images show the DNA marker DAPI in blue, H3 S10p in magenta, and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual H3 S10p channels. Within each mutant, we looked at GFP⁻ clones within the zone of non-proliferation of the wing disc to determine whether there was a consistent increase of H3 S10p incorporation, which might indicate an increase of mitotic cells. Conversely, we looked at GFP⁻ clones outside of the zone of non-proliferation to determine whether there was a consistent decrease of H3 S10p incorporation compared to their neighboring GFP⁺ control cells. c Merged images show the DNA marker DAPI in blue, TUNEL in magenta, and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the individual TUNEL channels. Within each mutant genotype, we looked at GFP⁻ clones outside the edges of the disc to determine whether there was a consistent increase of TUNEL compared to their neighboring GFP⁺ control cells