Fig. 2

Mutation of H3 K115, T118 and K115/K122 and K56Q causes growth defects in imaginal discs. a–k ×40 images of wing imaginal discs with GFP-negative mutant clones generated using heat-shock FLP at 72 h AEL. Merged images show the nuclear marker DAPI and GFP⁺ and GFP⁻ regions demarcate histone wild-type cells and histone mutant cells, respectively. Grayscale images are the GFP channels individually, shown for contrast. Note the smaller size of most GFP⁻ mutant areas compared to their GFP⁺⁺ twin spot. ΔHisC mutant clones cannot survive. l Graph representing the quantification of average clone area per ×40 wing disc image. Asterisk represents significance at p < 0.05, which was calculated using a two-sample t test with wild type as the reference group