Fig. 9

MYC2 distribution at the promoters of RD29B and RD29A in response to dehydration stress and to treatment with JA. ChIP–qPCR analysis of FLAG-MYC2 levels measured by FLAG-specific antibodies and the promoter regions of RD29B and at RD29A genes in transgenic plants expressing the FLAG-MYC2 fusion protein. A FLAG-MYC2 levels at RD29B in response to a first and a repeated dehydration stresses in plants untreated with JA. FLAG-MYC2 levels at RD29B upon exposure to JA, after the removal of JA and in response to the first dehydration stress, are shown on the right-hand side. Specific primers used for the recovered DNA are from the promoter (region 1) as indicated in Fig. 8. Background levels for immunoprecipitated samples with IgG for each amplicon are shown below; B FLAG-MYC2 levels at RD29A in response to dehydration stresses in plants untreated and treated with JA. Annotations, conditions, and IgG profiles are as described above for RD29B. The experiments were repeated three times, each with three replicates, and the representative experiments shown indicate the mean ± SE, n = 3 replicates. Letters above bars indicate significant difference among the treatments in the regions of interest (p < 0.05 according to Tukey’s multiple range test)