Fig. 6

Ser5Pol II levels at RD29B and RD29A in response to dehydration stress S1 in the absence of a functional MED25. Distribution and levels of Ser5Pol II at RD29A and RD29B determined by ChIP–qPCR assays with specific antibodies and amplified regions numbered as indicated on top. A, B Ser5Pol II levels at RD29B and RD29A measured in W and in S1 from wild type and med25 mutant plants without exposure to JA, respectively; C, d Ser5Pol II levels at RD29B and RD29A in W and in S1 from wild type and med25 mutant plants, respectively, in response to the treatment with JA. Experiments were repeated at least three times, each with three replicates, and the representative experiments shown indicate the mean ± SEM, n = 3 replicates. Letters above bars indicate significant difference among the treatments in the regions of interest (p < 0.05 according to Tukey’s multiple range test). Significant differences in Ser5Pol II levels in JA-untreated (S1) and JA-treated (S1J) samples are indicated by asterisks in A, C, according to Student’s t test