Fig. 4

Ser5P PolII and TATA binding protein (TBP) distribution profiles at RD29B and RD29A genes in response to dehydration stress and to treatment with JA. Ser5PPol II and TBP levels measured by ChIP–qPCR assays with specific antibodies under the various treatments, as annotated in Fig. 1B. Schematic diagrams of the genes are shown on top and annotated as in Fig. 3 above. Numbers below bars indicate regions assayed by ChIP. A Ser5 Poll II levels measured by ChIP–PCR at the indicated regions of RD29B; B Ser5 Poll II levels measured by ChIP-PCR at the indicated regions of RD29A; C distribution and TBP levels determined by ChIP-PCR at RD29B; D distribution and TBP levels determined by ChIP-PCR at RD29B. Numbers below show the locations of the regions analyzed by the ChIP assays. Experiments were repeated three times, each with three RT-qPCR measurements, and the representative experiment shown indicates the mean ± SEM, n = 3 replicates. For each point, letters above bars indicate significant difference among the treatments in the regions of interest (p < 0.05 according to Tukey’s multiple range test)