Fig. 2

Transcription patterns of dehydration stress response genes RD29B and RD29A upon a repeated dehydration stress with or without JA-pre-treatment. A Transcript levels of RD29B and of RD29A measured by real-time quantitative RT-PCR in response to two consecutive exposures to dehydration stress separated by recovery R1 and the effects of the treatment with JA. From here on, annotations under columns indicate treatment points as specified in Fig. 1b. B Transcript levels from the marker JA-responsive gene, TAT3, in response to a single (J1) and repeated exposures to JA (J2). C Transcript levels from RD29B and of RD29A genes in response to dehydration stress after experiencing two exposures to JA (S1J1 and S1J2). D Transcript levels of RD29B and of RD29A genes during shorter periods of exposure to JA and after 22-h recovery in the absence of JA. From here on, data for JA-treated samples are represented by slanted columns. qPCR data were normalized against ACT8 used as an internal control. Experiments were repeated three times, each with three qPCR measurements, and the results shown indicate the mean ± SEM, n = 3 replicates. Letters above the error bars indicate significant differences between stress responses (p < 0.05 according to Tukey’s multiple range test)