Fig. 6

DNA methylation and hydroxymethylation in chicken ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. A Immunodetection of 5-methylcytosine (a–e) and DNA counterstaining with propidium iodide (f–j) in nuclei of ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. B Immunodetection of 5-hydroxymethylcytosine (a–e) and DNA counterstaining with propidium iodide (f–j) in nuclei of ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. Single confocal images of representative nuclei are shown. Scale bar 5 μm. C Global levels of 5-methylcytosine and 5-hydroxymethylcytosine in chicken cells. 5mC and 5hmC levels were quantified by dot blot analysis of 125 ng of denatured genomic DNA from the different cell types; pBSK plasmid was used as a negative control. Error bars indicate the standard deviation of the mean signal for three technical replicates of one representative experiment