Fig. 3

Nuclear distribution of Polycomb group-deposited histone post-translational modifications in chicken ESCs and PGCs. Co-immunodetection of H2K119ub (a–c) and H3K27me2/3 (d–f), and DNA counterstaining with TO-PRO-3 (g–i) in nuclei of cESCs, RA-differentiated cESCs and PGCs. Overlay of H2K119ub (green) and H3K27me2/3 (magenta) is shown below (j–l). Single confocal images of representative nuclei are shown. Scale bar 5 μm