Fig. 2

Histone post-translational modifications of pericentric heterochromatin in chicken cells. A Co-immunodetection of H3K9me3 (a–e) and H3K27me2/3 (f–j), and DNA counterstaining with TO-PRO-3 (k–o) in nuclei of cESCs, RA-differentiated cESCs, BCs, PGCs and CEFs. White arrows indicate linescan and direction of intensity plots below, showing signal for H3K9me3 (blue), H3K27me2/3 (red) and TO-PRO-3 (grey) in the equatorial section of a typical nucleus. B Immunodetection of H3K27me2/3 (a) and DNA counterstaining by TO-PRO-3 (b) in nuclei in tissue sections from 9 day embryonic mesonephros. C Co-immunodetection of CENP-T (a, e) and H3K27me2/3 (c, g), and DNA counterstaining with TO-PRO-3 (b, f) in nuclei of cESCs and RA-differentiated cESCs. Overlay of CENP-T (white), H3K27me2/3 (magenta) and TO-PRO-3 (blue) is also shown (d, h). Single confocal sections of representative nuclei are shown. Scale bar 5 μm