Fig. 8

SIN3 and dKDM5/LID bind to common gene targets. Real-time quantitative PCR analysis of chromatin prepared from indicated cell lines immunoprecipitated with beads conjugated to antibody to the HA tag or antibody to SIN3. a Enrichment of SIN3 220 at predicted target genes. b Enrichment of dKDM5/LID at target genes. Primers used in the PCR amplification of target regions spanning the TSS of indicated genes. CG31819 acts as a negative control. The results are the average of three biological replicates. c Enrichment of H3K9ac at promoter proximal regions of SIN3 and/or dKDM5/LID target genes upon knockdown of Sin3A, lid, or both under normal and oxidative stress conditions. d Enrichment of H3K4me3 at promoter proximal regions of SIN3 and/or dKDM5/LID target genes upon knockdown of Sin3A, lid, or both under normal and oxidative stress conditions. KD knockdown, P paraquat