Fig. 2

Knock down of lid leads to decreased cell proliferation in S2 cells and wing imaginal discs, similar to a reduction of Sin3A. a Quantification of cell density by cell counts of S2 cells treated with dsRNA targeting indicated proteins. Results are the average of five biological replicates. b Histogram of DNA content vs. cell counts for indicated cell lines by flow cytometry. Peaks for G1 and G2/M cell cycle phases are indicated in the top panel. c Control and Sin3A or lid knockdown wing disc clones generated using the Flip-out GAL4 system and immunostained with antibody to GFP. d Quantification of GFP signal in wing imaginal discs. Results are the average pixel counts from 20 wing imaginal discs. KD knockdown. ***, P < 0.001