Table 1 High-stringent nuclear RNAi-repressed heat-inducible genes (NHGs)

AC8.10

X

MuDR transposable element

AC8.10/11 and AC8.3/4 are two duplicated neighboring sequences

AC8.11

X

MuDR transposable element

AC8.3

X

MuDR transposable element

AC8.4

X

MuDR transposable element

B0491.2.1 (sqt-1)

II

No obvious transposable elements around

sqt-1 encodes a cuticle collagen; during larval and adult development, sqt-1 activity is required, likely redundantly, for normal cuticle, and hence organismal, morphology

C13B9.4a.1 (pdfr-1)

III

A short CER9 LTR in an intron

pdfr-1 encodes, by alternative splicing, three isoforms of a G-protein-coupled receptor (GPCR) required for normal locomotion

C13B9.4b.1

III

A short CER9 LTR in an intron

C13B9.4c.1

III

A short CER9 LTR in an intron

C18D4.6a

V

Several repeats, a short Tc1A at 3’ of the gene

C40A11.10

II

Several repeats around the gene

C44B12.6

IV

Several repeats around the gene

C44B12.6 is an ortholog of human IL25 (interleukin 25), IL17C (interleukin 17C), IL17B (interleukin 17B), IL17D (interleukin 17D), and IL17A (interleukin 17A); C44B12.6 is predicted to have cytokine activity, based on protein domain information

C49C3.12 (clec-197)

IV

No obvious transposable elements around

C-type LECtin

C50F4.3 (tag-329)

V

No obvious transposable elements around

tag-329 is an ortholog of human CTSL (cathepsin L), CTSV (cathepsin V) and CTSO (cathepsin O); tag-329 is predicted to have cysteine-type peptidase activity, based on protein domain information

C52E2.6 (fbxb-97)

II

No obvious transposable elements around

F-box B protein

F40D4.13

V

CER15

F41G4.5

X

CER12

F41G4.7

X

CER12

F55D10. 3 (glit-1)

X

No obvious transposable elements in the gene except some repeats around the gene

glit-1 is an ortholog of human TG (thyroglobulin)

F57G4.9

V

Repeat Turmoil2, Haringer Family

This gene was determined to be of Transposon in origin so has been supressed from the C. elegans protein set

F58H7.5

IV

CER3 (gypsy)

F58H7.7

IV

CER3 (gypsy)

F59B2.12

III

No obvious transposable elements around

M7.9

IV

No obvious transposable elements around

R09H3.3

X

CER12-I ~ 4 kb downstream

T10D4.4 (ins-31)

II

Several repeats around the gene

ins-31 encodes an insulin-like peptide of the insulin superfamily of proteins (OMIM: 176730, 147440); INS-31 is one of 38 insulin-like peptides in C. elegans, but is unique in that it contains three insulin repeats; although overexpression with INS-19 can result in low levels of larval lethality, the precise role of INS-31 in C. elegans development is not yet clear as INS-31/19 overexpression does not enhance dauer arrest in either a wild-type or daf-2 mutant background, and loss of INS-31 function does not result in a mutant phenotype

T11F9.10

V

CER15

Paralog of F40D.13, both overlaps with CER15

T11F9.18 (srh-1)

V

upstream to CER15

W08F4.9 (fbxb-14)

II

No obvious transposable elements in the gene except some repeats around the gene

fbxb-14 (F-box B protein)

Y37H2A.4 (fbxa-107)

V

CER3 ~ 500 bp upstream

F-box A protein. This gene encodes a protein containing an F-box, a motif predicted to mediate protein–protein interactions either with homologs of yeast Skp-1p or with other proteins; this gene’s encoded protein also contains an FTH/DUF38 motif, which may also mediate protein–protein interaction

Y38H6 C .5 (dct-10)

V

CER3-I in gene body, Tc4 right downstream

dct-10 (DAF-16/FOXO Controlled, germline Tumor affecting), dct-10 is predicted to have nucleic acid binding activity and zinc ion binding activity, based on protein domain information

Y43F4A.3

III

CER9

Y53F4B.10

II

Several repeats around the gene

Y53F4B.10 is orthologous to the human gene INSULIN (IRF4; OMIM: 176730), which when mutated leads to disease

Y56A3A.5

III

Several repeats in and around the gene

faah-5 is an ortholog of human FAAH (fatty acid amide hydrolase) and FAAH2 (fatty acid amide hydrolase 2); faah-5 is predicted to have carbon–nitrogen ligase activity, with glutamine as amido-N-donor, based on protein domain information

Y68A4A.13

V

Several repeats in and around the gene

Y77E11A.3

IV

Several repeats around the gene

Y79H2A.4

III

Several Repeat around the gene

Y79H2A.4 is an ortholog of human NDUFAB1 (NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1); Y79H2A.4 is predicted to have nucleotide binding activity, based on protein domain information

ZC15.1

V

CER12-I downstream

ZC15.10

V

CER12-I upstream

ZC15.3

V

CER12

ZK262.8

V

CER8

ZK262.9

V

CER8