Fig. 5

Endo-siRNA changes caused by hrde-1 mutation at different temperatures and generations. a Scatter plots comparing endo-siRNA levels of WT and hrde-1 mutant. The 12 generations were divided evenly into 4 phases (I to IV) chronologically. The normalized mean siRNA counts of three generations in each phase were used in this analysis for each protein-coding gene. Genes with increased siRNAs in the hrde-1 mutant over WT at each phase were highlighted in red (blue for ones with decreased siRNAs). b Chromosome distribution of genes with HRDE-1-dependent siRNA expression changes. c Scatter plots comparing mRNA levels of WT and hrde-1 mutant with genes with hrde-1(-)-dependent siRNA changes highlighted. d Box plot comparing mRNA expression levels between WT and hrde-1 mutant for genes with hrde-1(-)-dependent siRNA changes. e Box plot comparing siRNA expression between WT and hrde-1 mutant for all genes and genes with hrde-1-dependent mRNA changes. Only phase III (23C-G4, G5, and G6) results were plotted in panels c–e. See Additional file 7 for results of all four phases. Wilcoxon rank-sum test was used to calculate the p values