Fig. 2

Germline nuclear AGO protein HRDE-1 prevents heat activation in mRNA expression for a subset of its native target genes. a Scatter plots comparing the 15 and 23 °C transcriptomes for WT and hrde-1 mutant. Each dot corresponds to a protein-coding gene. Heat-induced genes and heat-repressed genes in WT or hrde-1 mutant, as well as high-stringent NHGs (nuclear RNAi-repressed heat-inducible genes) are labeled as indicated. b Venn diagram analysis of temperature-sensitive genes in WT and hrde-1 mutant. c Box plot analysis of HRDE-1-bound siRNAs for non-NHGs, NHGs, and high-stringent NHGs. 3XFLAG-HRDE-1 coIP siRNA sequencing data from [11] were used. Wilcoxon rank-sum test was used to calculate the p values. d Genomic distributions of NHGs, high-stringent NHGs, and LTR retrotransposons. e Number of NHGs located in the arms (1/4 of the chromosome length on each end) and central region (1/2 of chromosome length in the center) for each of the five autosomes. X chromosome was divided into two parts at the 1/6 of the length. p values were calculated by using the Chi-square test