Fig. 8

Endogenous H3.5 specifically accumulates around transcription start sites in human testicular cells. a Graphic representation of the distribution of endogenous H3.5 and H3.3 on the human genome. 5′-UTR: 5′-untranslated region. 3′-UTR: 3′-untranslated region. TSS: transcription start site. TES: transcription end site. b Aggregation plots of the endogenous H3.5 localization around TSSs. c Aggregation plots of the endogenous H3.3 localization around TSSs. Signals for genes with various expression levels are plotted against relative distance from TSS. d Gene expression-independent incorporation of endogenous H3.5 in human testis. The increment of each H3.3 and H3.5 ChIP-Seq signal intensity is shown with respect to the gene expression levels (per 10 %ile FPKM). The red and blue lines indicate the average increments of H3.3 and H3.5, respectively. The standard error (S.E.) of the signal intensities is indicated as a dotted line