Fig. 7
H3.5 exists in human testicular cells within seminiferous tubules. a–c Human testis sections immunohistochemically stained with the anti-H3.5 (a), anti-H3.1 (b), and anti-H3.3 (c) monoclonal antibodies. Bars indicate 50 μm. Arrows and arrow heads in the enlarged picture in panel (a) primary spermatocytes at leptotene and preleptotene stages, respectively. d Western blotting. Proteins from isolated sperm were separated by 15 % SDS-PAGE, transferred to a PVDF membrane, and probed with the anti-H3.5 monoclonal antibody (left panel) or the anti-H3 C-terminal peptide polyclonal antibody (right panel). Recombinant H3.5-H4 and H3.3-H4 complexes were used for controls. Asterisks represent the degraded H3