Fig. 6
The H3.5-specific antibody. a Western blot analysis of H3.5. Purified H3.1, H3.2, H3.3, H3T, and H3.5 were separated by 18Â % SDS-PAGE, and analyzed by Western blotting with the anti-H3.5 monoclonal antibody (left panel). The 18Â % SDS-PAGE gel stained with Coomassie Brilliant Blue is presented in the right panel. b Western blot analyses of endogenous H3.5 (left panel) and all H3Â s (right panel). Endogenous H3.5 and H3 proteins were detected by Western blotting with the anti-H3.5 monoclonal antibody (left panel) and the H3 C-terminus-specific antibody (right panel), respectively