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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis

Fig. 5

Mobility of GFP-H3.5 and GFP-H3.3 in living cells. a FRAP analysis of HeLa cells expressing GFP-H3.3 or GFP-H3.5. Bar indicates 10 μm. b The mobility of GFP-H3.3 or GFP-H3.5 in living cells, analyzed by bleaching one-half of the nucleus. The averages of the relative fluorescence intensity of the bleached area were plotted with the standard deviations (n = 10–11). c FRAP analysis of HeLa cells expressing GFP-H3.3 F104L or GFP-H3.5 L103F. Bar indicates 10 μm. d The mobility of the GFP-H3.3 F104L or GFP-H3.5 L103F mutant in living cells, analyzed by bleaching one-half of the nucleus. The averages of the relative fluorescence intensity of the bleached area were plotted with the standard deviations (n = 10–11)

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Epigenetics & Chromatin

ISSN: 1756-8935