Skip to main content
Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis

Fig. 4

Thermal stability of tetrasomes. a Purified tetrasomes containing H3.3 (lane 2), H3.3 F104L (lane 3), H3.5 (lane 4), and H3.5 L103F (lane 5) were analyzed by non-denaturing 6 % PAGE analysis. The gel was stained with ethidium bromide. Lane 1 represents the naked DNA used for the reconstitution of tetraosomes. b Histone compositions of the purified tetrasomes. Purified tetrasomes containing H3.3 (lane 2), H3.3 F104L (lane 3), H3.5 (lane 4), and H3.5 L103F (lane 5) were analyzed by 18 % SDS-PAGE with Coomassie Brilliant Blue staining. c The schematic representation of thermal stability assay. d The thermal stability curves for the tetrasomes containing H3.3 (open triangles), H3.3 F104L (closed triangles), H3.5 (open circles), and H3.5 L103F (closed circles) are presented. Three experiments were independently performed and averages of these results were plotted with standard deviation values

Back to article page