Fig. 3
Mutational analysis of H3.5 and H3.3. a Non-denaturing 6Â % PAGE analysis of the purified nucleosomes containing H3.5 and H3.3 mutants, stained with ethidium bromide. Lane 1 represents the naked DNA used in the nucleosome reconstitution. Nucleosome core particles are denoted by NCPs. b Histone compositions of the purified nucleosomes containing H3.3 and H3.5 mutants, analyzed by 18Â % SDS-PAGE with Coomassie Brilliant Blue staining. c Salt resistance assays of the H3.5 mutant nucleosomes, and d the H3.3 mutant nucleosomes. Bands corresponding to nucleosomes are indicated as NCPs. Asterisks represent bands corresponding to non-nucleosomal DNA-histone complexes [26]