Fig. 1
The nucleosome containing histone H3.5 is unstable. a Sequence comparison between human H3.1, H3.2, H3.3, H3T, and H3.5. The amino acids in H3.5 that differ from those in H3.3 are indicated by black boxes with white characters. The epitope peptide sequence used to generate the H3.5 antibody is underlined. The α-helices and β-strands found in the crystal structures of the human nucleosomes are represented on the top of the panel. b 18 % SDS-PAGE analysis of purified histones H3.1, H3.3, H3T, and H3.5, stained with Coomassie Brilliant Blue (CBB). c Non-denaturing 6 % PAGE analysis of purified nucleosomes containing H3.1, H3.3, H3T, and H3.5, stained with ethidium bromide. Lane 1 represents the naked DNA used in the nucleosome reconstitution. Nucleosome core particles are denoted by NCPs. d Histone compositions of the purified nucleosomes containing H3.1, H3.3, H3T, and H3.5, analyzed by 18 % SDS-PAGE with Coomassie Brilliant Blue staining. e Salt resistance assays of the H3.1 and H3.3 nucleosomes and f the H3.3, H3T, and H3.5 nucleosomes. Bands corresponding to nucleosomes are indicated by NCPs. Asterisks represent bands corresponding to non-nucleosomal DNA-histone complexes [26]