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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities

Fig. 3

Steady-state kinetics of SET7/9 methyl transfer activity to peptide derived from FoxO3 substrate. a Kinetic traces of SET7/9 at increased FoxO3 peptide concentrations (0–700 µM). Initial rates at each FoxO3 substrate concentration were calculated by fitting the raw data to a linear equation. Absorbance values at 340 nm in all reactions are normalized to 1 and the change in absorbance over time is presented as 1—the absorbance value obtains for each measurement. b Michaelis–Menten (MM) plots for SET7/9 activity with FoxO3 peptide. The kinetic parameters derived from the fit to the MM equations are k cat of 32.1 ± 5.4 min−1 and K M of 165.4 ± 20.2 μM

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