Fig. 5

Methylation state at ERV iiDMRs in liver is conserved in spleen. Shown is a UCSC genome browser screen shot of three variably methylated loci, iiDMR 24 (a), iiDMR 11 (b) and iiDMR 27 (c), on the Y-axis 0 represents no methylation, 1 represents 100 % methylation and the solid lines indicate the 50 % methylation position. Shown also are the underlying repeat elements. Clonal bisulphite sequencing (primer sequences are in Additional file 1: Table S5) from spleen revealed the same pattern of differential methylation across the five mice for ERV iiDMR 24 and for the two most extremes of methylation for ERV iiDMR 11 and ERV iiDMR 27. Each sample is represented by at least seven clones, filled in circles represent methylated CpGs. Asterisk indicates T test, p value <0.05