Fig. 4

Four distinct modes of nucleosome remodeling. Representative signal profiles of k-means clusters belonging to each mode of remodeling (a central; b non-central; c phased; d minimal). deltaH3K4methyls (red), GR (green), and deltaMNase (black) signals are overlaid for comparison. deltaMNase signal is magnified eightfold to facilitate visual comparison. Heatmaps illustrate nucleosome positioning prior to GR binding (NoStim H3K4methyls signal). Red box overlying heatmap of cluster 12 highlights evidence of well-positioned, phased nucleosomes in the unstimulated condition