Fig. 2

Definition and validation of deltaH3K4methyls signal. a Snapshot demonstrating how deltaH3K4me2 track is defined—normalized H3K4me2 ChIP-Seq signal from unstimulated cells is subtracted from that of dexamethasone-stimulated cells, emphasizing the pattern of ChIP-Seq signal change at this GR binding site. b Snapshots demonstrating how the deltaH3K4methyls track is defined. At many sites, the summed signal has the same shape as its components (upper panel), while at others the deltaH3K4methyls track resolves discordancies between component signals (lower panel). c Aggregate signal profile of normalized H3K4me2 ChIP-Seq signal from unstimulated (solid blue) and dexamethasone-stimulated (solid red) cells in a 3-kb window surrounding all GR summits. Normalized MNase-Seq signal from unstimulated (dashed blue) and dexamethasone-stimulated (dashed red) cells is overlaid. d Aggregate signal profile of deltaH3K4me2 (blue) versus deltaH3K4methyls (red) versus deltaMNase (black) in a 3-kb window surrounding all GR summits