Fig. 5

Biased X-inactivation occurs upon HAT release in clonal cell populations harbouring two-active X-chromosomes. Scatterplots of expression levels from the paternal (x-axis) relative to the maternal (y-axis) X-chromosome for the indicated gene in individual cells of the indicated clones assessed by allelic RT-qPCR (BioMark, Fluidigm). Each dot represents a single TS cell. (+HAT): cells at passage 6 maintained under HAT pressure; (−HAT): cells after HAT removal. The same samples of cells have been analysed for the expression of the genes shown in this figure and in Additional files 8 and 9. F3: n = 77; 3#C (+HAT): n = 75; 2#C (+HAT): n = 108; 1#C & 3#C (−HAT): n = 144; 1#A & 2#C (−HAT): n = 141. (See Additional file 7) for raw quantifications. A.U. Arbitrary Units