Skip to main content
Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: A rapid passage through a two-active-X-chromosome state accompanies the switch of imprinted X-inactivation patterns in mouse trophoblast stem cells

Fig. 1

HAT-resistant clones of F3 female TS cells show various patterns of Xist expression including an inverted Xist expression profile. a Protocol of isolation of HAT-resistant TS clones. Female F3 TS cells were either directly treated with HAT or pre-treated with 6-TG before HAT selection. HAT-resistant clones were picked at day 10 of selection and maintained under HAT selection for 2 additional passages. Analysis of Xist expression was performed after 8 passages. b Table showing the frequencies of HAT-resistant clones obtained in the time course of 6 independent experiments (#A, #B, #C, #D, #E and #F). The frequency of HAT-resistant clones after pre-treatment with 6-TG is significantly different from the frequency of HAT-resistant clones in the absence of 6-TG pre-treatment (Mann–Whitney test, p value <0.05). The asterisk indicates that in experiment #C, the HAT selection has been maintained for a longer period of time, see Additional file 5A. The protocol described in panel a has been applied in the other experiments (i.e. #A, #B, #D, #E, and #F). In this study, each individual clone analysed is identified by a capital letter which refers to the experiment from which the clone has been isolated and by a number which identifies each clone of a given experiment. Clones that have been characterised further in this study all come from HAT selection without 6-TG pre-treatment. c Cumulative histograms showing the Xist expression levels measured by allelic RT-qPCR after standardisation by the Rplp0 reporter gene [20] in the 40 clones obtained in experiment #F (passage 8 after clone picking). Paternal and maternal expressions are depicted in blue and red above and below the x-axis, respectively. For F3 parental female cells, mean and standard deviation have been calculated on the basis of three independent cell cultures. A.U. Arbitrary Units

Back to article page