Fig. 2

Distribution of DHSS in VPA- or vehicle-treated visual cortex. a Average DHSS tag enrichment around CAGE promoters from a previous study [31] from vehicle (Veh, solid line) and VPA-treated visual cortex (dotted line). Average TPM (tags per million) are computed in 500 bp bins centered around the TSS. Promoters located on chrM are excluded, as are promoters containing a bin with average TPM higher than 10 times the average TPM for that bin across all promoters. b Venn diagram showing the distribution of DHSS tags. c Validation of DHSS by qPCR. DNase I digested DNA from VPA- or vehicle-treated visual cortex were amplified with primer pairs annealing within induced DHSS proximal or distal to CAGE TSS clusters, or randomly in the genome. Data from two biological replicates for each treatment condition is shown (VPA 1 and 2, Veh 1 and 2). Error bars are standard deviations. d Genomic distribution of DHSS clusters with respect to RefSeq gene models