Fig. 10

Distinct radial positioning of gene-poor and gene-dense segments of chromosomes 1 and 12 with regard to the border of granulocyte nuclear lobes. a ideograms of chromosomes 1 and 12 with regional gene density (left bar). Localization of individual BAC clones representing either gene-dense (green) or gene-poor segments (red) used in this study is marked by an asterisk. b left panel: respective 2D-FISH control experiments with the expected banded pattern on metaphase chromosomes; right panel: z-projections of 3D-FISH experiments. c 3D measurements for the distance distributions of signals delineating gene-dense (green) and gene-poor (red) segments, respectively, reveal their significantly distinct radial distribution both for chromosome 1 (top) and for chromosome 12 (bottom); (p < 0.005 for both curves, assessed by Mann–Whitney rank sum test). The ordinate denotes the normalized sum of voxel intensities for a respective fluorochrome, the abscissa the relative distance to the nuclear border. Nuclear counterstain (DAPI) is denoted in blue. n number of analyzed nuclei; error bars standard error of means