Fig. 5
From: Ethnicity-specific epigenetic variation in naïve CD4+ T cells and the susceptibility to autoimmunity
Using a luciferase reporter vector with an inserted promoter/5'-untranslated regions from IL32 shows a significant difference in luciferase expression between fully methylated and unmethylated regions as measured by a ratio of Lucia luciferase over firefly luciferase luminescence. A cartoon (a) of the linearized plasmid showing the location of the IL32 promoter/5'-untranslated region inserts in the Lucia Luciferase reporter vector and the number of CpG sites present in each insert. The expression of the Lucia luciferase reported as a ratio of Lucia/firefly luciferase (b)