Fig. 2
From: The histone chaperone DAXX maintains the structural organization of heterochromatin domains
Disruption of chromocentres in the absence of DAXX. Correlative LM-ESI micrographs. H3K9me3 fluorescence (left panel, top) overlaid on the mass sensitive image generating the correlative image (left panel, bottom). High magnification ESI micrograph of the region shown in the white box (correlative image) of the corresponding H3K9me3-containing structure (arrowhead). Approximate boundaries of the H3K9me3 region are indicated by a dashed line. a Non-nucleolar and b perinucleolar heterochromatin domain of wild-type cell. c–g H3K9me3 correlative LM-ESI micrographs of DAXX null cells. (c, e, and g) Non-nucleolar and (d and f) perinucleolar heterochromatin structures. In all ESI images, chromatin is represented by levels of yellow and protein-based structures as cyan. Nu nucleolus. Scale bar 0.5 µm. h Quantification of the number of typical non-nucleolar and perinucleolar H3K9me3-containing heterochromatin domains in wild type and DAXX null cells