Fig. 1
From: The histone chaperone DAXX maintains the structural organization of heterochromatin domains
The association of H3.3 with H3K9me3-enriched chromatin is increased in the absence of DAXX. a Wild type and DAXX null fibroblasts immunolabeled for H3.3 (red) and H3K9me3 (green). Line scan intensity plots of H3.3 and H3K9me3 for magnified chromocentres, H3.3 associated (I, III) and non-associated (II, IV) chromocentres. Scale bar 5 µm. b Quantification of the percent association of H3.3 and H3K9me3-enriched chromatin domains in wild type (n = 52) and DAXX null (n = 54) cells. Data calculated as the mean percent of association per cell. c Quantification of the average number of chromocentres (H3K9me3-enriched foci). Error bars represent SEM from three independent experiments. d, e Western blot analysis of whole-cell lysates of H3.3 (d) and H3K9me3 (e)