Fig. 2

EtOH exposure in vitro alters levels of transcripts encoding Sod3 and Tet1, but does not impact measures of cell death or oxidative stress. a Measurements of transcripts encoding proteins involved in the metabolic processing of alcohol and oxidative stress response pathways. Primary neuroepithelial stem cells were cultured in the presence of 160 or 240 mg/dL EtOH for 3 days, followed by a 4-day recovery in media lacking EtOH. Samples were harvested at days-3 and 7, and transcript levels determined by RT-qPCR. Graphs represent three independent biological replicates (N = 3), with two independent RT reactions and three independent qPCR measurements for each RT. Significance was measured using a one-way ANOVA, error bars represent SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. b–e Measures of cellular stress and apoptosis in primary neuroepithelial stem cells exposed to alcohol. Cells were cultured in the presence of 80–240 mg/dL EtOH for 3 days, then assayed for markers of b and c apoptosis, d oxidative stress and e cellular stress. Differences were measured using a one-way ANOVA, error bars represent SEM. Graphs represent three separate biological replicates (N = 3)