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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects

Fig. 1

Dose-dependent epigenetic signatures of EtOH exposure persist past the period of exposure. a Experimental paradigm. b Alcohol-induced epigenetic alterations in H3K4 me3, H3K9 ac, H3K9 me2, and H3K27 me3. Primary fetal cerebral cortical neuroepithelial stem cells were cultured in the presence of 160 or 240 mg/dL EtOH for 3 days, followed by a 4-day recovery in medium lacking EtOH. Samples were collected at days 3 and 7, and examined for changes in the indicated post-translational histone modifications using chromatin immunoprecipitation followed by quantitative PCR (ChIP-qPCR). Heat maps represent fold change in H3K4 me3, H3K9 ac, H3K9 me2, and H3K27 me3 within the regulatory regions of the genes listed. Primers were designed to fall within 250 base pairs of the transcriptional start site. Within the three separate biological replicates (N = 3), three ChIPs were performed, and two qPCR replicates performed on each independent ChIP. Statistical measures were conducted using the Wilcoxon signed rank nonparametric test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

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