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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression

Fig. 1

Generation of comprehensive epigenomic maps of histone modifications at three different stages of intra-erythrocytic stages of P. falciparum life cycle. a Schematic representation of different stages of Plasmodium falciparum harvested at 18, 30 and 40 h post-synchronization. A single large-scale culture of highly synchronized cells was used for ChIP-sequencing of all histone modifications. b A snapshot of the representative genome browser shows the histone methylation and acetylation, and RNA sequencing reads at trophozoite stage. Representative active chromatin (presence of RNA-seq reads), inactive chromatin (presence of H3K9me3 and absence of RNA-seq reads) and poised chromatin (present of histone activation marks but no RNA-seq reads) are highlighted in green, red and blue color, respectively. Anti-IgG antibody was used as a negative control. c Heat map representing the correlation between occupancy of indicated histone modifications in the gene body for all P. falciparum genes. Active promoter marks; H3K4me2, H3K4me3, H3K9ac and H3K14ac clustered separately to form an active chromatin state (green square). Histone variant H2A.z and other histone modifications; H4K20me3, H3K79me3, H3K36me3, H3K27ac, H3K4me1 and H4ac are clustered together to form active/poised chromatin state (blue square). H3K9me3 and H3K36me2 are anti-correlated with each other suggesting mutually exclusive function for these two histone modifications

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