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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Tissue-specific expression of histone H3 variants diversified after species separation

Fig. 5

Histone variants correlate with two differentiation states. a Principal component analysis of gene expression patterns in H3 variant-overexpressing C2C12 cell differentiation. The x- and y-axes indicate the PC1 and PC2 scores, respectively, of the gene expression profiles in each cell. Blue points indicate the growth state; red points indicate the differentiated state. The distance between two points reflects the dissimilarity in gene expression patterns between cells. The higher PC1 score (PC1+) indicates a higher expression of muscle differentiation-related genes and lower expression of cell growth-related genes, as illustrated in the top bar. Similarly, higher PC2 scores (PC2+) indicate higher expression of ER-stress-related genes and lower expression of extracellular matrix (ECM)-related genes. Clusters of cells that have similar gene expression patterns, G (green), D1/2 (blue) and D3 (purple), are highlighted. b Gene expression levels of each H3 variant confirmed by RT-PCR amplicon-Seq. Expression levels of representative genes chosen from the top four contributors (genes) for each PC direction (PC1± and PC2±) are shown as boxplots calculated from three replicates. The illustration below shows the order of each H3 variant-expressing cell line in the growth and differentiated state. The color of each box corresponds to the expression groups shown in a: blue, undifferentiated wild-type (WT); red, differentiated WT; green, growth (G); blue, D1/2; and purple, D3. Two-sided Student’s t test was performed on group-average expression levels between “WT” vs. “Differentiated” for PC1± genes, and between “D1/2” vs. “D3” groups for PC2± genes

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