Fig. 2

H3 variant genes are expressed in mouse tissues. a Representative IGV screenshots of 3′-seq data of the four tissues. The mRNA sequences mapped in four tissues (brain, liver, skeletal muscle and testis) are shown in four lanes at each locus of the H3 variants. The black thick bars indicate exons, and black thin bars indicate the predicted 3′-UTR of each H3 variant gene. The predicted lengths of the 3′-UTRs are shown below the genes. Piled-up depths are shown as (min–max) to the left of each lane. b The gene expression levels of novel H3 variants in four tissues. The expression levels of H3 variants (log₁₀ RPM) are indicated by the color bar to the right. Tiles marked with white asterisks confirm H3 variant expression (RPM > 0) in the replicated data. c Identified peptides and their positions in histone sequences. LC–MS/MS analysis was performed using acid extracts from adult male mouse tissues (8 week old), as shown in Additional file 2: Figure S8A. Red letters mark amino acids specific to H3t and H3mm7. Red underline indicates unique peptide sequences. Green, yellow and light blue indicate peptides in the peptide sequence with high confidence, medium confidence and low confidence, respectively