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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Tissue-specific expression of histone H3 variants diversified after species separation

Fig. 1

Identification of novel mouse H3 variants. a Schematic drawing of the H3 variant gene screening by in silico hybridization. We first split known H3 (H3.2) amino acid sequences into short fragments of eight amino acids and listed all possible combinations of fragments with respect to the codon table. Next, DNA sequences were mapped onto the mouse genome. Adjacently mapped sequences (<90 bp) were concatenated, and the concatenated regions were considered H3 variant coding genes. b Protein sequences of novel H3 variants. The protein sequences were translated from predicted H3 variant genes. Characteristic amino acids are in red type. The blue highlighted region indicates position 31 and the green highlighted region indicates amino acids at the N-terminal tail and the motif that discriminate H3.1 and H3.3. c Clusters of similar mouse H3 variant proteins. The cluster dendrogram was constructed by hierarchical clustering of H3 variant amino acid sequences. The edit distance (Levenshtein distance) was used as a similarity measure. Height (y-axis) indicates the edit distance between cluster pairs. Clusters containing H3.1 or H3.3 were defined as H3.1 and H3.3 groups, respectively. Other clusters distant from the H3.3 sequence were defined as distant groups A or B

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